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Tripotent Lgr5 stem cells in the posterior tongue generate non-taste lingual, taste and salivary gland cells [scRNA-seq]

GSE274015 Mus musculus Expression profiling by high throughput sequencing 5 samples Submitted 2025/09/01 Platform GPL24247
Summary
The circumvallate papillae (CVP) and foliate papillae (FoP) of the posterior tongue contain taste buds in close proximity to specialized salivary glands, known as von Ebner and minor salivary glands, respectively. The developmental relationship between taste buds and these salivary glands remains largely unexplored. Lineage tracing studies in mice have revealed that Lgr5 marks taste bud stem cells. Here, we report single-cell RNA sequencing of the entire CVP and FoP of mice, yielding transcriptional profiles of cells from tongue surface epithelium, taste buds and the associated salivary glands. We unveiled a developmental trajectory in which taste buds, the associated salivary glands and the non-taste tongue surface epithelium originate from a common Lgr5+ cell. We describe long-term organoid culture conditions for these cells and confirm their tripotency at the clonal level in vitro. CVP and FoP harbor chemosensory units consisting of taste bud and salivary gland cells derived from the same parental Lgr5+ stem cell.
Published in
Tripotent Lgr5 stem cells in the posterior tongue generate lingual, taste, and salivary gland lineages
Verweij LHG, Kim SY, Laskaris D et al. · Nature communications 2025 · PMID 41271704 · doi:10.1038/s41467-025-65140-0
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Direct links to NCBI, no account and no request form: the whole study as GSE274015_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 5 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1144471 and SRA study SRP524506. Searching any of these in the dataset finder brings you back here.

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