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Multi-omics characterization of human Embryonic Stem Cells-derived Pharyngeal Endoderm cells (Single-cell RNA-Seq Batch_2)

GSE274133 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2024/08/10 Platform GPL20301
Summary
We have developed a novel in vitro protocol for the derivation of bona fide Pharyngeal Endoderm (PE) cells from hESCs. We demonstrated that our PE cells robustly express Pharyngeal Endoderm markers, they are transcriptionally similar to PE cells isolated from in vivo mouse development and represent a transcriptionally homogeneous population. Importantly, we elucidated the contribution of Retinoic Acid in promoting a transcriptional and epigenetic rewiring of PE cells. In addition, we defined the epigenetic landscape of PE cells by combining ATAC-Seq and ChIP-Seq of histone marks.
Published in
Transcriptional and epigenetic characterization of a new in vitro platform to model the formation of human pharyngeal endoderm
Cipriano A, Colantoni A, Calicchio A et al. · Genome biology 2024 · PMID 39118163 · doi:10.1186/s13059-024-03354-z
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Also filed as BioProject PRJNA1145015 and SRA study SRP524624. Searching any of these in the dataset finder brings you back here.

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