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A TGF-β-induced long non-coding RNA eSNAI1 activates SNAI1 enhancer in cis and stimulates epithelial to mesenchymal transition of cancer cells

GSE274181 Homo sapiens Expression profiling by high throughput sequencing 9 samples Submitted 2025/03/06 Platform GPL28038
Summary
We identified a tumor-promoting long non-coding RNA (lncRNA) named eSNAI1 (enhancer RNA of SNAI1) which is induced by TGF-beta and enforces TGF-beta signaling. It was found to promtotes TGF-beta-induced epithelial-to-mesenchymal transition (EMT), cell migration and extravasation in a zebrafish exnograft model. To better dissect the downstream target genes and elucidate the signaling pathways altered by eSNAI1, we overexpressed eSNAI1 by CRISPRa in MDA-MB-231 cells. Total RNA was collected and sent for RNA sequencing analysis. Afterwards, differentially expressd genes were used for the pathway enrichment analysis and gene set enrichment analysis (GSEA).
Published in
Identification of a SNAI1 enhancer RNA that drives cancer cell plasticity
Fan C, Wang Q, Krijger PHL et al. · Nature communications 2025 · PMID 40133308 · doi:10.1038/s41467-025-58032-w
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Also filed as BioProject PRJNA1145363 and SRA study SRP524867. Searching any of these in the dataset finder brings you back here.

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