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Npac regulates pre-mRNA splicing in mouse embryonic stem cells

GSE274429 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2024/08/16 Platform GPL24247
Summary
As a reader of tri-methylated lysine 36 on histone H3 (H3K36me3), Npac has been shown a significant role in gene transcription elongation. However, its potential implication in RNA splicing remains unknown. Here, we characterized the phenotypes of Npac knockout in mES cells. We discovered that loss of Npac disrupts pluripotency and identity in mESCs. We also found that Npac is associated with many cellular activities including cell proliferation, differentiation, transcription regulation. Notably, we uncovered that Npac is associated with RNA splicing machinery. Furthermore, we found that Npac regulates alternative splicing through its interaction with the splicing factors including Srsf1. Our research thus highlights the important role of Npac in maintaining ESC identity through regulation of pre-mRNA splicing.
Published in
Npac Regulates Pre-mRNA Splicing in Mouse Embryonic Stem Cells
Qian Y, Ye Y, Zhang W et al. · International journal of molecular sciences 2024 · PMID 39408725 · doi:10.3390/ijms251910396
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Also filed as BioProject PRJNA1146403 and SRA study SRP525404. Searching any of these in the dataset finder brings you back here.

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