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Single-cell transcriptomic profiling of brain immune infiltration following Venezuelan equine encephalitis virus infection

GSE274566 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2024/12/09 Platform GPL30172
Summary
Neurotropic alphaviruses such as Venezuelan equine encephalitis virus (VEEV) are critical human pathogens that continually expand to naïve populations and for which there are no licensed vaccines or therapeutics. The neuropathology of VEEV has been attributed to the immune response in the brain yet the underlying mechanisms and specific immune cell populations involved are not fully elucidated. In this study, a murine model of lethal VEEV infection is paired with single-cell RNA sequencing to transcriptionally profile the immune response longitudinally in the brain following infection. Infection-induced immune changes in the brain was also compared to changes in peripheral blood mononuclear cells (PBMCs). The results define an inflammatory response involving transcriptionally distinct subpopulations of activated microglia and infiltrating proinflammatory myeloid populations and cytotoxic lymphocytes. This study advances our understanding of the immune mechanisms underlying viral encephalitis toward the goal of identifying new therapeutic targets.
Published in
Single-cell and spatiotemporal transcriptomic profiling of brain immune infiltration following Venezuelan equine encephalitis virus infection
Rangel MV, Sebastian A, Leon NF et al. · Frontiers in immunology 2024 · PMID 39749347 · doi:10.3389/fimmu.2024.1497839
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Direct links to NCBI, no account and no request form: the whole study as GSE274566_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1147221 and SRA study SRP526268. Searching any of these in the dataset finder brings you back here.

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