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Single-cell RNA-seq of Bronchoalveolar Lavage Fluid Immune Cells from Padi2/Padi4 Double Knock-out Mice in Pseudomonas aeruginosa Pneumonia-induced Sepsis

GSE274823 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2024/11/30 Platform GPL21103
Summary
Sepsis-induced acute lung injury (ALI) is prevalent in septic patients and has a high mortality rate. Considering ALI’s close link to sepsis, we used a Pseudomonas aeruginosa (PA) pneumonia-induced sepsis mouse model to investigate alveolar microenvironment alterations and lung injury post-sepsis. Peptidyl arginine deiminase (PADI) 2 and PADI4 are highly expressed in immune cells, and play substantial roles in the immune response to sepsis, but their specific functions remain unclear.We employed single-cell RNA sequencing (scRNA-seq) technology to map immune cell populations in bronchoalveolar lavage fluid (BALF) cells from Wild type (WT) and Padi2 and Padi4 double knock-out (DKO)mice in PA pneumonia-induced sepsis and sham conditions.
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Direct links to NCBI, no account and no request form: the whole study as GSE274823_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1148132 and SRA study SRP530129. Searching any of these in the dataset finder brings you back here.

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