← BioTransfer GEO Dataset Finder
GEO series

ZAR1/2-regulated Histone Modifications are Required for Zygotic Activation and Age-associated Oocyte Quality Maintenance

GSE275156 Mus musculus (mouse) ChIP-seq / ATAC-seq (genome binding and open chromatin) 12 samples Submitted 2025/03/05 Platform GPL34290
At a glance

ChIP-seq / ATAC-seq (genome binding and open chromatin) · mouse · 12 samples

What it is
ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
Organism
Mus musculus (mouse)
Samples
12 samples: 6 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
ZAR1/2-Regulated Epigenetic Modifications are Essential for Age-Associated Oocyte Quality Maintenance and Zygotic ActivationAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
Summary (written by the submitters)
The developmental competence and epigenetic progression of oocytes gradually become dysregulated with increasing maternal age. However, the mechanisms underlying age-related epigenetic regulation in oocytes remain poorly understood. Zygote arrest proteins 1 and 2 (ZAR1/2) are two maternal factors with redundant roles in maintaining oocyte quality.
Read the full summary ↓
Here, we found that Zar1/2-deleted oocytes exhibited reduced levels of multiple histone modifications and of the corresponding histone modifiers, along with over-condensed chromatin, leading to disordered histone modification, compromised zygotic genome activation and deficient embryo development following fertilisation. Cytoplasmic ZAR1/2 participated in intranuclear epigenetic maturation by binding to the transcripts that encoding histone modifiers in oocytes, and regulating their stability and translational activity. Moreover, oocytes from older mice exhibited similar histone-modification deficiency to Zar1/2-deleted oocytes. In contrast, forced ZAR1/2 expression in aged mouse oocytes partially restored histone modifications. ZAR1/2 mRNA and protein levels were lower in oocytes from older mice and in those from older women, suggesting ZAR1/2 as regulators of aging-associated epigenetic disorganizations. This study presents a new nucleo-ooplasmic interaction mechanism that is vital for oocyte epigenetic maturation and declining of developmental potentials with female aging. Further, it provides the potential gene targets for diagnosis and clinical intervention in age-associated deficiency in oocyte and embryo development.
Published in
ZAR1/2-Regulated Epigenetic Modifications are Essential for Age-Associated Oocyte Quality Maintenance and Zygotic Activation
Rong Y, Wu YK, Chen Y et al. · Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 · PMID 39755931 · doi:10.1002/advs.202410305
This dataset
Open GSE275156 in finder → Find more mouse ChIP / ATAC / CUT&Tag datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE275156_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.

Also filed as BioProject PRJNA1149704 and SRA study SRP527305. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 39755931) ↗
Study design
6 conditions, mostly in duplicate
ATAC_WT_GO ×2 ATAC_KO_GO ×2 ATAC_WT_GV ×2 ATAC_KO_GV ×2 ATAC_WT_L1C ×2 ATAC_KO_L1C ×2

Supports a between-group comparison across 12 samples.

How we read this: 6 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all mouse ChIP / ATAC / CUT&Tag datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.