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Tonicity drives maturation of the collecting ducts in the mammalian kidney

GSE275292 Mus musculus (mouse) RNA-seq (gene expression) 16 samples Submitted 2026/07/15 Platform GPL20795Platform GPL21273
At a glance

RNA-seq (gene expression) · mouse · 16 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
16 samples: 13 distinct titles, 2 of them repeatedGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Organ maturation is a fundamental question in biology and a key requisite for organoid maturation in regenerative medicine. As the kidney matures, the osmolality in the renal medulla increases for urine concentration, but how this extreme environment conversely contributes to kidney maturation is unclear. Here we show that high tonicity, i.e.
Read the full summary ↓
high salt concentration, promotes the maturation of mouse embryonic kidneys and human kidney organoids. Exposure of mouse embryonic collecting ducts (CDs) to high tonicity induces morphological changes in the epithelia, upregulates maturation-associated genes, and confers responsiveness to antidiuretic hormone, which mainly depend on the transcription factor NFAT5 (nuclear factor of activated T cells 5). Based on these findings, we have established a robust maturation protocol for human CD organoids that allows recapitulation of disease states caused not only by NFAT5 deletion, but also by a missense mutation of the antidiuretic hormone receptor reported to cause congenital nephrogenic diabetes insipidus. Collectively, high tonicity is a driving force for mammalian kidney maturation, not only for urinary concentration. Our study uncovers a novel principle of organ maturation triggered by environmental cues, and offers a promising path to maximize the potential of organoids for future therapies.
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE275292_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1150209 and SRA study SRP527594. Searching any of these in the dataset finder brings you back here.

Study design
13 distinct titles, 2 of them repeated
MK P ×2 P7_medulla ×2

How we read this: Read from the first 15 of 16 sample titles: 13 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 1 more — browse all 16 samples with per-sample file links →

Datasets on a similar topic

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.