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Single cell RNA-seq reveals GINIP-Expressing neurons as the main targets of focused ultrasoud.

GSE275643 Mus musculus (mouse) Single-cell RNA-seq 57 samples Submitted 2025/03/01 Platform GPL21103
At a glance

Single-cell RNA-seq · mouse · 57 samples

What it is
Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart.
Organism
Mus musculus (mouse)
Samples
57 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Focused ultrasound (US) is a recently-explored method for neurostimulation. One potential advantage of focused US compared to electrical stimulation is the ability to selectively target restricted subpopulations of neurons. This feature makes US neurostimulation a promising alternative tool for the design of future neural prostheses: for example, to restore hearing in deaf people, because contemporary cochlear implants – which work via electrical stimulation - mainly suffer from a lack of spatial selectivity.
Read the full summary ↓
Focused US was recently shown to directly stimulate neurons in several in vitro and in vivo studies; however the mechanisms of this effect still remain poorly understood. The aim of this interdisciplinary PhD project is to understand the physical and molecular causes of US-induced neurostimulation. The molecular mechanisms will be identified by combining US, calcium imaging and single-cell RNA sequencing on cultured dorsal root ganglia (DRG) neurons. The physical mechanisms (thermal and mechanical effects of US action) will be measured using temperature monitoring and laser vibrometry and confronted to numerical simulations of the in vitro set-up.
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE275643_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA1152354 and SRA study SRP528584. Searching any of these in the dataset finder brings you back here.

Study design
A cohort of 57 individual samples

The grouping that defines any comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Sample titles run NWSN92 … NWSN174 and carry no group information.

Samples in this study

+ 17 more — browse all 57 samples with per-sample file links →

Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.