← BioTransfer GEO Dataset Finder
GEO series

GeoMx DSP- profiling of mouse spleen

GSE275724 synthetic construct; Mus musculus Spatial transcriptomics 67 samples Submitted 2025/12/31 Platform GPL17021Platform GPL19604
At a glance

Spatial transcriptomics · synthetic construct; Mus musculus · 67 samples

What it is
Spatial transcriptomicsGene activity measured with its position in a tissue section.
Organism
synthetic construct; Mus musculus
Samples
67 samples: 8 conditions, mostly with about 12 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
B cells drive CD4 T cell immunosenescence and age-associated health declineScience immunology · 2026
Summary (written by the submitters)
GeoMx DSP- profiling of mouse spleen
Published in
B cells drive CD4 T cell immunosenescence and age-associated health decline
Khan S, Chakraborty M, Wu F et al. · Science immunology 2026 · PMID 41616067 · doi:10.1126/sciimmunol.adv7615
This dataset
Open GSE275724 in finder → Find more RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE275724_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Spatial studies usually ship Space Ranger style folders (count matrix plus tissue images). Look for them among the supplementary files.

Also filed as BioProject PRJNA1152792 and SRA study SRP528820. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41616067) ↗
Study design
8 conditions, mostly with about 12 replicates each
DSP-1001660013460-E-E ×12 DSP-1001660013460-E-F ×12 DSP-1001660013460-E-G ×12 DSP-1001660013460-E-D ×9 DSP-1001660013460-E-C ×7 DSP-1001660013460-E-A ×5 DSP-1001660013460-E-B ×5 DSP-1001660013460-E-H ×5

Supports a between-group comparison across 67 samples.

How we read this: 8 replicated groups read from 67 sample titles; they account for 67 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 27 more — browse all 67 samples with per-sample file links →

Popular RNA-seq datasets

No close match found for this topic, so these are simply the most-viewed datasets of the same kind.

Search all RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.