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Maintenance of histone crotonylation by YEATS2 and GCDH drives EMT in head and neck cancer [RNA-seq]

GSE275977 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2025/07/15 Platform GPL24676
Summary
Regulation of gene expression is an integral cellular process, fine-tuned by epigenetic marks like histone modifications. Perturbations in the activity or abundance of epigenetic factors can lead, or contribute to tumorigenesis in healthy cells. Remarkably, the addition of epigenetic marks is dependent on the metabolites produced during several essential cellular pathways. Here, we have explored the interplay between a highly expressed epigenetic factor YEATS2, and a metabolic enzyme GCDH in regulating epithelial to mesenchymal transition in head and neck cancer. We report the results of RNA-seq in YEATS2-silenced BICR10 cells to investigate the effect of YEATS2 in influencing gene expression globally. We have also performed ChIP-seq using YEATS2 antibody in BICR10 cells to obtain the globally occupied sites of YEATS2. Lastly, we report the changes in crotonylation at histone 3 lysine 27 (H3K27Cr) upon YEATS2 downregulation in BICR10 by performing ChIP-seq in shControl vs. shYEATS2 cells.
Published in
Interplay of YEATS2 and GCDH regulates histone crotonylation and drives EMT in head and neck cancer
Pant D, Kakani P, Joshi R et al. · eLife 2025 · PMID 40810390 · doi:10.7554/eLife.103321
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Direct links to NCBI, no account and no request form: the whole study as GSE275977_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1154005 and SRA study SRP529499. Searching any of these in the dataset finder brings you back here.

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