Topology regulates cell differentiation in situ

To study the topology regulation of cell differentiation in situ, mesh-like flap spacers with different diameters and spacing were prepared by near-field electrostatic printing. Firstly, the structural characteristics and biocompatibility of the materials were studied. The effect of the flap shim prepared by in vivo near-field electrostatic printing technology on skin tissue was analyzed by mRNA-seq, and the related genes and proteins were detected by flow cytometry, qPCR, and Western blotting to clarify the mechanism of the topological structure on the flap. Finally, it was explored in animals.