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Single-cell RNA-sequencing of angioblasts and endothelial cells from E9.5-E11.5 mouse embryos

GSE276281 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2024/10/18 Platform GPL19057
Summary
Background: The formation of the lymphatic system has been a subject of controversy for over a century. Although the accepted view is that lymphatic endothelial cells originate exclusively from veins, there have been reports of non-venous derived lymphatics in multiple tissues. Methods: We performed single-cell RNA sequencing analyses on ~20000 individual cells isolated from Pax3Cre/+;Rosa26tdTomato mouse embryos at E9.5, E10.5 and E11.5, the temporal window in which lymphatic endothelium initially emerges. Results: These analyses revealed the existence of populations of lymphangioblasts which arise from paraxial mesoderm and directly incorporate into the lymph sacs. Our findings support the notion that lymphatic endothelial cells also have non-venous origins and explain the discrepancies between previous studies.
Published in
Direct specification of lymphatic endothelium from mesenchymal progenitors
Lupu IE, Grainger DE, Kirschnick N et al. · Nature cardiovascular research 2025 · PMID 39747454 · doi:10.1038/s44161-024-00570-5
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Also filed as BioProject PRJNA1156232 and SRA study SRP530319. Searching any of these in the dataset finder brings you back here.

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