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Analyzing CREB binding motif and sites in the mouse lens epithelial cells alpha-TN4 overexpressing Vector, WT-CREB, S133A-CREB, R314A-CREB and S133A/R314A –CREB.

GSE276462 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 6 samples Submitted 2025/02/05 Platform GPL28330
Summary
Transcription factor CREB regulates lens Epithelial-Mesenchymal Transition(EMT)in both S133 phosphorylation dependent and independent manners. We found that S133A-CREB can activate lens EMT, while R314A-CREB attenuated it. To understand the mechanism by which S133A-CREB and R314A-CREB modulates EMT gene expression. ChIP assay using CREB antibody in the in the mouse lens epithelial cells alpha-TN4 overexpressing Vector, WT-CREB, S133A-CREB, R314A-CREB and S133A/R314A-CREB, and ChIP-seq was conducted on the ChIP DNA. We found that WT-CREB, S133A-CREB as well as R314A-CREB binds to the promoter regions of mesenchymal genes, and S133A-CREB retained canonical CREB binding motif, while R314A-CREB exhibited shifted binding motif preference to ATF1.
Published in
The transcription factor CREB regulates epithelial-mesenchymal transition of lens epithelial cells by phosphorylation-dependent and phosphorylation-independent mechanisms
Zhang L, Wang JM, Wang L et al. · The Journal of biological chemistry 2025 · PMID 39662835 · doi:10.1016/j.jbc.2024.108064
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Also filed as BioProject PRJNA1157419 and SRA study SRP530713. Searching any of these in the dataset finder brings you back here.

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