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IWS1 Stimulates Pol II Transcription Elongation In Vivo [RNA-Seq]

GSE276547 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2025/07/30 Platform GPL21697
Summary
Interacts with SPT6 1 (IWS1) has been implicated in various co-transcriptional processes, yet its direct role in RNA Polymerase (Pol) II transcription in vivo remains elusive. In this study, we utilized a rapid depletion system of IWS1 in human cells, followed by multi-omics analysis and biochemical assays, to elucidate its function. We show that IWS1 depletion results in a global decrease of RNA synthesis, but does not affect the chromatin recruitment of the histone methyltransferase SETD2. In vivo and in vitro analyses of nascent transcription and histone modifications revealed that the observed decrease in methylation is a secondary effect of the transcription defect caused by IWS1 depletion. Whereas these results show that IWS1 is essential for maintaining normal RNA Pol II elongation velocity in vivo, and that it can directly stimulate transcription.
Published in
IWS1 positions downstream DNA to globally stimulate Pol II elongation
Zheenbekova A, Walshe JL, Ochmann M et al. · Nature communications 2025 · PMID 40835814 · doi:10.1038/s41467-025-62913-5
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Also filed as BioProject PRJNA1157818 and SRA study SRP531088. Searching any of these in the dataset finder brings you back here.

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