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Hydrogen sulfide preserves intestinal barrier repair function through sulfhydration of RPS20 in experimental colitis

GSE276745 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2025/03/21 Platform GPL24676
Summary
Patients with ulcerative colitis (UC) have a significantly impaired intestinal barrier. Hydrogen Sulfide (H2S) is a gaseous mediator that makes notable contributions in a variety of diseases, such as reducing inflammatory response in colitis. The experimental content includes the establishment of a mouse DSS-induced colitis model, mouse colon epithelial organoids culture, H&E staining and mass spectrometry analysis. We recognized that exogenous H2S donor-GYY4137 significantly alleviated the symptoms in UC mice models and maintained Minichromosome Maintenance Complex Component 2 (MCM2) expression. CBS knockdown reduced the expression of sulfhydrated Ribosomal protein S20 (RPS20-ssh) and MCM2 in the mouse colon. Cell experiments indicated that the expression of RPS20-ssh, rather than total expression of RPS20, is responsible.
Published in
Hydrogen sulfide preserves intestinal barrier repair function through sulfhydration of RPS20 in experimental colitis
He X, Li J, Huang Z et al. · Scientific reports 2025 · PMID 40399407 · doi:10.1038/s41598-025-02268-5
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Also filed as BioProject PRJNA1158786 and SRA study SRP531577. Searching any of these in the dataset finder brings you back here.

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