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Comparison of Different Approaches to Single Cell RNA Sequencing of Cancer Associated Fibroblasts

GSE276754 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2025/09/14 Platform GPL24247
Summary
Cancer associated fibroblasts (CAFs) represent a major stromal component of PDAC with both tumor-promoting and restraining properties. Studies have demonstrated functional heterogeneity among different subpopulations of CAFs, highlighting the need to identify specific subpopulations when targeting CAFs. We hereby used two types of processing method, whole tumor or negatively-isolated CAFs, to determine how best to uncover CAF heterogeneity with single-cell RNA sequencing (scRNA-seq).
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Direct links to NCBI, no account and no request form: the whole study as GSE276754_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1158804 and SRA study SRP531623. Searching any of these in the dataset finder brings you back here.

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