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Study of MED1 acetylation in ER positive breast cancer cells [RNA-Seq II]

GSE276763 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2025/08/01 Platform GPL30173
Summary
Chromatin occupancy of MED1 and other Mediator components, as well as Pol II, were studied in MCF7 cells. MED1 acetylation affects chromatin occupancy of its related factors, which was analyzed with CUT&RUN-seq, ChIP-seq and sequential ChIP-seq assay (by pan-acetyl-lysine antibody) with MED1 KR mutant (HA tag) where acetylation residues were replaced. Combined with RNA seq analysis for gene expression in MCF7 cells with MED1 KO and KR/KQ MED1 ectopic expression, the role of MED1 acetylation in gene transcription control was investigated.
Published in
MED1 IDR deacetylation controls stress responsive genes through RNA Pol II recruitment
Lin R, Mo Y, Barrows D et al. · Nature chemical biology 2026 · PMID 41131199 · doi:10.1038/s41589-025-02035-7
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Also filed as BioProject PRJNA1158822 and SRA study SRP531647. Searching any of these in the dataset finder brings you back here.

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