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Selective interactions at pre-replication complexes categorize baseline and dormant origins

GSE276856 Homo sapiens (human) ChIP-seq / ATAC-seq (genome binding and open chromatin) 211 samples Submitted 2025/04/06 Platform GPL30173
At a glance

ChIP-seq / ATAC-seq (genome binding and open chromatin) · human · 211 samples

What it is
ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
Organism
Homo sapiens (human)
Samples
211 samples: 96 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Selective interactions at pre-replication complexes categorize baseline and dormant originsNature communications · 2025
Summary (written by the submitters)
The initiation of DNA synthesis in metazoans is restricted to a group of baseline replication origins, whereas other (dormant) origins do not initiate replication despite recruiting apparently indistinguishable pre-replication complexes. Dormant origins are activated as backups when DNA synthesis stalls. We report that dormant origins selectively bind CDK-phosphorylated RecQL4 (pRecQL4), a helicase mutated in Rothmund-Thomson, RAPADILINO and Baller-Gerold syndromes.
Read the full summary ↓
In unperturbed cells, pRecQL4 restricts replication initiation to baseline origins by preventing dormant origins from binding the essential initiation factor MTBP. When cells encounter replication stress, pRecQL4 first promotes the dissociation of MTBP from chromatin, and then enables its redistribution to both baseline and dormant origins, facilitating compensatory initiation to complete genome duplication. Thus, MTBP-pRecQL4 interactions modulate replication origin choice and facilitate recovery from replication stress.
Published in
Selective interactions at pre-replication complexes categorize baseline and dormant origins
Thakur BL, Redon CE, Fu H et al. · Nature communications 2025 · PMID 40319014 · doi:10.1038/s41467-025-59509-4
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE276856_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.

Also filed as BioProject PRJNA1159299 and SRA study SRP531943. Searching any of these in the dataset finder brings you back here.

Study design
96 conditions, mostly in duplicate
AS, MTBP, ×2 AS, pMCM2, ×2 AS, pRQ4, ×2 DTB, 0hr, MTBP, ×2 DTB, 0hr, pMCM2, ×2 DTB, 0hr, pRQ4, ×2 DTB, 1hr, MTBP, ×2 DTB, 1hr, pMCM2, ×2 +107 more

Supports a between-group comparison across 192 samples.

How we read this: 96 replicated groups read from 211 sample titles; they account for 192 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 171 more — browse all 211 samples with per-sample file links →

Datasets on a similar topic

Search all human ChIP / ATAC / CUT&Tag datasets in GEO →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.