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Long read direct RNA-sequencing of human monocyte derived macrophages for chimeric RNA discovery

GSE277057 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2026/08/02 Platform GPL26167
Summary
Classically, mammalian mRNA and protein are thought to be encoded from a single genetic locus known as a gene. Whether mRNA can contain exons from geographically distinct genes to form chimeric mRNA in the absence of malignancy is widely unknown. Pervasive and naturally occurring chimeric mRNA could greatly expand the functional diversity of mammalian transcriptomes and potentially generate novel hybrid proteins that underpin diverse biological processes. Here we demonstrate that long-read direct RNA-seq, paired with targeted mRNA splice-isoform analysis, enables the identification of a plethora of dynamically regulated chimeric mRNA in innate immune cells.
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Direct links to NCBI, no account and no request form: the whole study as GSE277057_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1160301 and SRA study SRP532346. Searching any of these in the dataset finder brings you back here.

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