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Transcriptome changes caused by PHF6 knockout in THP1 human AML cell line

GSE277149 Homo sapiens Expression profiling by high throughput sequencing 9 samples Submitted 2024/09/18 Platform GPL18573
Summary
CRISPR Cas9 guided knockout (KO) of PHF6 in human THP1 AML cell line. We performed bulk RNA-Seq on knockout (PHF6 KO) and wildtype (CTRL) clones derived from THP1 cells transduced with lentiviral vectors encoding Cas9 protein and either PHF6 gRNAs or non-targeting gRNAs. Our results reveal that PHF6 knockout upregulates self-renewal gene sets and downregulates myeloid differentiation gene sets.
Published in
PHF6 suppresses self-renewal of leukemic stem cells in AML
Jalnapurkar SS, Pawar AS, George SS et al. · Leukemia 2024 · PMID 39004675 · doi:10.1038/s41375-024-02340-5
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Also filed as BioProject PRJNA1160872 and SRA study SRP532593. Searching any of these in the dataset finder brings you back here.

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