CUT&RUN sequencing on wildtype HSCs, MPP3s, GMPs, and macrophages with four weeks M. avium infection

Purpose: Central trained immunity is a phenomenom in which reprogrammed HSPCs differentiate into innate immune cells with heightened immune responses. To determine whether histone modifications are conserved and passed down to downstream lineage cells, we performed CUT&RUN sequencing on various HSPC subpopulations and macrophages in both M. avium infected and naive states. This was done to determine if there were conserved changes across all subpopulations, which would imply to us if specific gene signatures and genomic loci could be conserved for eliciting central trained immunity.