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SFSWAP is a negative regulator of OGT intron detention and global pre-mRNA splicing [RNA-seq I]

GSE277950 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2024/10/16 Platform GPL30173
Summary
In this study, we identified SFSWAP as a negative regulator of O-GlcNAc transferase (OGT) intron 4 detention and global pre-mRNA splicing using a CRISPR knockout screen in an O-GlcNAc responsive GFP reporter cell line. In order to characterize the functions of the protein, we performed siRNA mediated knockdown of SFSWAP followed by RNA-seq analysis under untreated conditions. Alternate splicing analysis using rMATS (and other tools) revealed enhanced splicing of retained introns and increased inclusion of cassette exons upon knockdown of SFSWAP, supporting an inhibitory role for SFSWAP in splicing.
Published in
SFSWAP is a negative regulator of OGT intron detention and global pre-mRNA splicing
Govindan A, Conrad NK · bioRxiv : the preprint server for biology 2025 · PMID 39464125 · doi:10.1101/2024.10.14.618288
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Also filed as BioProject PRJNA1164794 and SRA study SRP534516. Searching any of these in the dataset finder brings you back here.

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