GEO series

Tet2 loss leads to increased hematopoietic stem cell self-renewal and myeloid transformation

GSE27816 Mus musculus (mouse) Expression microarray 14 samples Submitted 2011/07/01 Platform GPL1261

At a glance

Expression microarray · mouse · 14 samples

What it is: Expression microarrayGene activity read on a chip (the older technology that RNA-seq replaced).
Organism: Mus musculus (mouse)
Samples: 14 samples
Data you can get: A processed expression table (series matrix) and the array files, from NCBI.

Summary (written by the submitters)

Recurrent somatic mutations in TET2 and in other genes that regulate the epigenetic state have been identified in patients with myeloid malignancies and in other cancers. However, the in vivo effects of Tet2 loss have not been delineated. We report here that Tet2 loss leads to increased stem-cell self-renewal and to progressive stem cell expansion.

Read the full summary ↓

Consistent with human mutational data, Tet2 loss leads to myeloproliferation in vivo, notable for splenomegaly and monocytic proliferation. In addition, haploinsufficiency for Tet2 confers increased self-renewal and myeloproliferation, suggesting that the monoallelic TET2 mutations found in most TET2-mutant leukemia patients contribute to myeloid transformation. This work demonstrates that absent or reduced Tet2 function leads to enhanced stem cell function in vivo and to myeloid transformation. These studies show that a ubiquitin ligase-substrate pair can orchestrate the molecular program of HSC differentitiation

This dataset

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Download · which file do I need?

Direct links to NCBI, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need.

All supplementary files ↗

3 · The original raw files

Whole study · RAW.tar

Everything the authors deposited as one archive (GSE27816_RAW.tar), including the raw array or instrument files. Can be large; the picker in step 2 takes just the samples you need.

Whole study · RAW.tar ↓

What the files usually look like: For arrays the series matrix is normally the normalised expression table you want; the raw array files (CEL and similar) are in the RAW.tar.

Also filed as BioProject PRJNA137933. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 21723200) ↗

Samples in this study

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+ 14 more — browse all 14 samples with per-sample file links →

Datasets on a similar topic

GSE278208 STING Mediates Increased Self-Renewal and Lineage Skewing in DNMT3A-Mutated Hematopoietic Stem/Progenitor Cells 40 samples shares hematopoietic, self-renewal, increased
GSE264041 TET2 Deficiency Increases the Competitive Advantage of Hematopoietic Stem Cells through Upregulation of Thrombopoietin Receptor Signaling 28 samples shares tet2, hematopoietic
GSE190226 Concomitant loss of TET2 and TET3 results in T cell expansion and genomic instability [BGI-RNA-Seq] 21 samples shares tet2, loss
GSE243492 MALAT1 is required for phenotypic consequences of TET2 mutations in myeloid neoplasms. 12 samples shares tet2, myeloid
GSE266580 Ablation of Id1 Reduces Clonal Hematopoiesis, Genomic Instability and Delays Leukemia in Mice with Tet2 Loss of Function [RNA-seq] 12 samples shares tet2, loss
GSE233879 Increased molecular and functional heterogeneity of hematopoietic stem cells contributes to age-related decline of tissue and body function 80 samples shares hematopoietic, increased
GSE262396 Mitochondria-enriched hematopoietic stem cells exhibit elevated self-renewal capabilities, thriving within the context of aged bone marrow [bulk RNA-seq] 24 samples shares hematopoietic, self-renewal
GSE296499 CK2α deletion in the hematopoietic compartment leads to mild alterations in terminally differentiated cells and expansion of stem cells 22 samples shares hematopoietic, leads

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