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Co-profiling of in situ RNA-protein interactions and transcriptome in single cells and tissues [long reads]

GSE278262 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2025/06/02 Platform GPL34382
Summary
RNA-binding proteins (RBPs) are essential regulators of RNA fate and function. A long-standing challenge in studying RBP regulation has been mapping RNA interactomes within the dynamic transcriptomic landscape, especially in single-cell contexts and primary tissues. Here we introduce MAPIT-seq (modification added to RBP interacting transcript-sequencing), which uses an antibody-directed editing strategy to map genome-wide in situ RBP–RNA interactions and gene expression concurrently. We demonstrate MAPIT-seq’s robustness across multiple RBPs and systematically analyze RNA substrates associated with core polycomb repressive complex 2 (PRC2) components. MAPIT-seq is also applicable to frozen tissue sections, enabling the mapping of RBP roles during brain development. Importantly, we develop high-throughput single-cell MAPIT-seq (scMAPIT-seq) to reveal cell stage-specific RBP regulation. In summary, MAPIT-seq expands multi-omics profiling, providing an effective framework to study post-transcriptional regulation in dynamic biological processes and clinically relevant scenarios.
Published in
Co-profiling of in situ RNA-protein interactions and transcriptome in single cells and tissues
Cheng QX, Xie G, Zhang X et al. · Nature methods 2025 · PMID 40784921 · doi:10.1038/s41592-025-02774-4
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Also filed as BioProject PRJNA1166190 and SRA study SRP535377. Searching any of these in the dataset finder brings you back here.

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