← BioTransfer GEO Dataset Finder
GEO series

Single-cell RNA transcriptome reveals embryonic origin of offspring fibrosis due to maternal obesity

GSE278631 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2024/12/12 Platform GPL24247
Summary
Over 40% of pregnant American women are obese which negatively affects fetal development with long-term consequences for offspring health. Maternal obesity (MO) leads to fibrotic infiltration in skeletal muscle, brown adipose tissue, and other tissues/organs of offspring, but mechanisms remain to be defined. Using a mouse model of diet-induced obesity and unbiased single-cell RNA-sequencing (scRNA-seq), we tested the effeCONs of MO on embryonic fibrogenesis, which serves as the source of fibrogenic and stromal cells in adults. Embryonic somatic tissues were obtained at E9.5, E11.5 and E13.5 of control (CON) and MO mice. Using unsupervised clustering of scRNA-seq data, we identified three major cell clusters with increasing fibrogenic capacity. Compared to CON, the population of fibrogenic cells increased dramatically due to MO, correlated with the elevated expression of transforming growth factor β (Tgfb) and paired related homeobox 1 (Prrx1), as well as fibrogenic genes.
Published in
Corrigendum to "Single-cell RNA transcriptomics in mice reveals embryonic origin of fibrosis due to maternal obesity" [eBioMedicine 109(2024) 105421]
Hossain MN, Gao Y, Li X et al. · EBioMedicine 2025 · PMID 39874704 · doi:10.1016/j.ebiom.2025.105573
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE278631_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1168119 and SRA study SRP536260. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 8 more — browse all 8 samples with per-sample file links →

Similar datasets

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.