Identification of lncMB3 RNA interactome through lncMB3 Native RNA Pulldown in MYC-amplified D283 Med cells.

Medulloblastoma (MB) stands as the most prevalent malignant pediatric tumor affecting the central nervous system. Categorized into four primary subgroups, each with distinct biological and clinical features, MB presents unique therapeutic implications. Group 3 (G3) and 4 (G4) MBs collectively account for the majority of cases (60%) and exhibit the highest degree of aggressiveness. However, these subgroups remain poorly characterized from a biochemical perspective. Therefore, it is crucial to unveil the fundamental molecular mechanisms underlying G3 e G4 MBs to deepen our overall comprehension of these specific conditions and to pave the way for precision medicine-driven approaches. Compared to normal human cerebella, the MYC-dependent lncRNA MB3 was found to be induced in G3-derived cell lines expressing the proto-oncogene and upregulated in G3-MB primary tumors. Consistently, LncMB3 is downregulated upon MYC inhibition and in vitro studies reveal its potent anti-apoptotic activity. To deepen the study of the LncMB3 molecular mechanism, we employed native RNA Pulldown assays followed by RNA-Sequencing to identify LncMB3 interactome. Here we provide the data of polyA+ RNA samples derived from two biological replicates of LncMB3 Native RNA Pulldown sample sets (INPUT, EVEN, ODD, LacZ) derived from the MYC-amplified D283 Med cell line that were subjected to RNA-Seq analysis.