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CCAAT/enhancer-binding proteins alpha and beta directly regulate ovulation and selectively regulate gene expression in granulosa cells by preovulatory stage- and dose-dependent mechanisms

GSE279171 Mus musculus (mouse) RNA-seq (gene expression) 18 samples Submitted 2025/05/05 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 18 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
18 samples: 6 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
CCAAT/Enhancer-Binding Proteins α and β Regulate Ovulation and Gene Expression via Dose- and Stage-Dependent MechanismsEndocrinology · 2025
Summary (written by the submitters)
The preovulatory luteinizing hormone surge orchestrates complex cellular and molecular events leading to ovulation. CCAAT/enhancer-binding proteins alpha and beta (C/EBPα/β) are transcription factors acutely induced by the luteinizing hormone surge and crucial for ovulation and granulosa cell luteinization. However, biological processes and their regulatory mechanisms downstream of C/EBPα/β in the preovulatory ovary are not completely understood.
Read the full summary ↓
To address this knowledge gap, we generated Cebpa/bfl/fl;Pgr-Cre mutants and compared them with Cebpa/bfl/fl;Cyp19a1-Cre mutant female mice. The Cebpa/bfl/fl;Cyp19a1-Cre mutants have undetectable levels of C/EBPα/β throughout preovulatory stages and no ovulation, aligning with previous reports, while Cebpa/bfl/fl;Pgr-Cre mutants presented gradual deletion of C/EBPα/β during late preovulatory stage and reduced ovulation rate. Comparison of these two models indicates that sustained expression of C/EBPα/β throughout preovulatory stages is necessary for successful ovulation. This provides a unique opportunity to interrogate gene regulatory mechanisms by C/EBPα/β during different preovulatory time windows and the impact of dysregulating C/EBPα/β on ovulation-associated biological processes. Our study revealed that C/EBPα/β regulate gene expression and distinct biological functions via preovulatory stage-, dose-, and/or potentially isoform ratio- dependent mechanisms. These findings shed new light on the intricate gene regulation mechanisms by C/EBPα/β downstream of the LH surge.
Published in
CCAAT/Enhancer-Binding Proteins α and β Regulate Ovulation and Gene Expression via Dose- and Stage-Dependent Mechanisms
Zhang H, Lanz RB, Dhillon J et al. · Endocrinology 2025 · PMID 40294160 · doi:10.1210/endocr/bqaf081
This dataset
Open GSE279171 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE279171_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1169875 and SRA study SRP536992. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40294160) ↗
Study design
6 conditions, mostly in triplicate
mouse ovarian granulosa cells, WT, 8 h post-h… ×3 mouse ovarian granulosa cells, Cebp-C, 8 h po… ×3 mouse ovarian granulosa cells, Cebp-P, 8 h po… ×3 mouse ovarian granulosa cells, WT, 12 h post-… ×3 mouse ovarian granulosa cells, Cebp-C, 12 h p… ×3 mouse ovarian granulosa cells, Cebp-P, 12 h p… ×3

Supports a between-group comparison across 18 samples.

How we read this: 6 replicated groups read from 18 sample titles; they account for 18 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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