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Genetic landscape and functional exploration of kidney cancer predisposition causality in cross-ancestral populations

GSE279473 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2026/02/10 Platform GPL24676
Summary
To functionally explore renal cell carcinoma (RCC) susceptibility variants, we performed CUT&Tag for H3K27ac and ATAC-seq on 786-O cells to assess transcriptional activity. Subsequently, we conducted a CRISPR screen and CRISPR droplet sequencing (CROP-seq), which combines pooled CRISPR screens with single-cell RNA sequencing (scRNA-seq), to identify target genes potentially regulated by likely causal SNPs. Functionally, we established a novel association between rs28684409 and the oncogene RPL4 at the complex genetic locus 15q22.31. As the next step, we performed CRISPRi on rs28684409 and conducted RNA-seq to identify differential gene expression associated with this variant.
Published in
Genetic landscape and functional exploration of kidney cancer predisposition in cross-ancestral populations
Dai H, Chu X, Du H et al. · Nature communications 2026 · PMID 42000752 · doi:10.1038/s41467-026-71785-2
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Also filed as BioProject PRJNA1173091 and SRA study SRP538622. Searching any of these in the dataset finder brings you back here.

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