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Microstructures regulate distant cellular interactions during heart formation

GSE279629 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2024/12/01 Platform GPL21626
Summary
Communication between endocardium and myocardium, separated by cardiac jelly, is essential for cardiac morphogenesis. How membrane-localized receptors and ligands achieve interaction across the cardiac jelly is not understood. Utilizing a number of approaches, including genetic labeling of cell-cell contacts, we found that myocardium and endocardium interact directly via microstructures, termed Tunneling Nanotube-Like microstructures (TNTLs). TNTLs extend from cardiomyocytes to directly contact endocardial cells (ECs). TNTLs transport cytoplasmic proteins, transduce signals between cardiomyocytes and ECs, and initiate myocardial growth toward the lumen to form ventricular trabeculae-like structures. Loss of TNTLs disturbs signaling interactions and, subsequently, ventricular patterning.
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Also filed as BioProject PRJNA1173733 and SRA study SRP538951. Searching any of these in the dataset finder brings you back here.

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