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Transcriptome analysis of the course of ischemic stroke from acute to chronic phase in mice [2]

GSE279666 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2026/02/20 Platform GPL30172
Summary
We conducted an examination of alterations in gene expression in the ischemic brain of mice at 3 and 14 days after cerebral infarction, in comparison to the control and sham operation groups at single nucleus-transcriptomic levels. By cell type annotation, we obtained neurons (glutamatergic and GABAergic), fibroblast-like cells, astrocytes, oligodendrocyte progenitor cells (OPCs), oligodendrocytes, microglia, endothelial cells, and pericytes. We observed survival signals in neurons in addition to extensive inflammatory changes and neuronal death signals. A massive change was observed on day3, showing the loss of glutamatergic neuron and the increase of endothelial cells. This tendency was slightly recovered towards day14. Our findings revealed elevated expression of autophagy and cAMP signaling in surviving neurons within the penumbra region.
Published in
Characterizing stroke-related cellular changes in the surviving neurons of mouse ischemic stroke
Ikegami T, Sasaki T, Shimbo T et al. · Neurochemistry international 2025 · PMID 41223896 · doi:10.1016/j.neuint.2025.106086
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Also filed as BioProject PRJNA1174054 and SRA study SRP539083. Searching any of these in the dataset finder brings you back here.

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