Effect of genetic deletion of Gsdmd on mice fed an oxalate rich-diet

The role of pyroptosis, an inflammatory form of cell death involving the formation of Gasdermin-D (GSDMD) pores, in chronic oxalate nephropathy remains unknown. We report genetic deletion of Gsdmd exacerbated oxalate nephropathy in mice in association with accelerated necroptosis.Our transcriptional analysis using kidneys from mice fed an oxalate-rich diet or control diet revealed significant changes in the gene sets related to leukocyte migration, chemokine pathways, mitochondrial dysfunction, and complement pathways between Gsdmd KO and WT mice. These differences may be attributed to variations in the substances released during cell death.