← BioTransfer GEO Dataset Finder
GEO series

mRNAseq profiling of hCoV229E infected A549 cells

GSE280154 Homo sapiens (human) RNA-seq (gene expression) 30 samples Submitted 2026/02/02 Platform GPL24676
At a glance

RNA-seq (gene expression) · human · 30 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
30 samples: 10 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
NRF2 activators and the inhibitor of nuclear export, selinexor, restrict coronaviruses by targeting a network involving ACE2, TMPRSS2, and XPO1 through an NRF2-independent mechanismCommunications biology · 2026
Summary (written by the submitters)
A549 cells are infected with hCoV229E and treated with Bardoxolone, 4-octyl itaconate and Selinexor in order to understand the antiviral mechanism of these treatments
Published in
NRF2 activators and the inhibitor of nuclear export, selinexor, restrict coronaviruses by targeting a network involving ACE2, TMPRSS2, and XPO1 through an NRF2-independent mechanism
Waqas FH, Silva da Costa L, Zapatero-Belinchón FJ et al. · Communications biology 2026 · PMID 41721027 · doi:10.1038/s42003-026-09724-6
This dataset
Open GSE280154 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE280154_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1176558 and SRA study SRP540437. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41721027) ↗
Study design
10 conditions, mostly in triplicate
A549 cells control 24h ×3 A549 cells hCoV229E infected 24h ×3 A549 cells hCoV229E infected+ Bardoxolone tre… ×3 A549 cells hCoV229E infected+ 4-octyl itacona… ×3 A549 cells hCoV229E infected+ selinexor treat… ×3 A549 cells control 48h ×3 A549 cells hCoV229E infected 48h ×3 A549 cells hCoV229E infected+ Bardoxolone tre… ×3 +2 more

Supports a case/control comparison: 24 samples read as cases, 6 as controls.

How we read this: 10 replicated groups read from 30 sample titles; they account for 30 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.