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p300/CBP-catalyzed acetylation safeguards minor zygotic genome activation via activating DUX [ChIPseq_ATAC-seq_Stacc-seq]

GSE280521 Mus musculus (mouse) ChIP-seq / ATAC-seq (genome binding and open chromatin) 50 samples Submitted 2025/05/12 Platform GPL24247
At a glance

ChIP-seq / ATAC-seq (genome binding and open chromatin) · mouse · 50 samples

What it is
ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
Organism
Mus musculus (mouse)
Samples
50 samples: 24 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
DUX-mediated configuration of p300/CBP drives minor zygotic genome activation independent of its catalytic activityCell reports · 2025
Summary (written by the submitters)
Maternal-deposited factors initiate zygotic genome activation (ZGA), driving the maternal-to-zygotic transition; however, the coordination between maternal coactivators and transcription factors (TFs) in this process remains unclear. In this study, by profiling the dynamic landscape of p300 during mouse ZGA, we reveal its role in promoting RNA polymerase II (Pol II) pre-configuration at ZGA gene regions and sequentially establishing enhancer activity and regulatory networks.
Read the full summary ↓
Moreover, p300/CBP-catalyzed acetylation drives Pol II elongation and minor ZGA gene expression by inducing pivotal TFs such as Dux. Remarkably, the supplementation of exogenous Dux rescues ZGA failure and developmental defects caused by the loss of p300/CBP acetylation. DUX functions as a pioneer factor, guiding p300 and Pol II to minor ZGA gene regions and activating them in a manner dependent on the non-catalytic functions of p300/CBP. Together, our findings reveal a mutual dependency between p300/CBP and DUX, highlighting their coordinated role in regulating minor ZGA activation.
Published in
DUX-mediated configuration of p300/CBP drives minor zygotic genome activation independent of its catalytic activity
Xiao L, Jin H, Dang Y et al. · Cell reports 2025 · PMID 40202846 · doi:10.1016/j.celrep.2025.115544
This dataset
Open GSE280521 in finder → Find more mouse ChIP / ATAC / CUT&Tag datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE280521_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.

Also filed as BioProject PRJNA1158345 and SRA study SRP535417. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40202846) ↗
Study design
24 conditions, mostly in duplicate
p300_L1C_DMSO ×2 p300_L1C_A485 ×2 p300_E2C_DMSO ×2 p300_E2C_A485 ×2 p300_E2C_A485+Dux ×2 p300_L2C_DMSO ×2 p300_L2C_A485 ×2 p300_L2C_A485+Dux ×2 +18 more

Supports a between-group comparison across 48 samples.

How we read this: 24 replicated groups read from 50 sample titles; they account for 48 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 10 more — browse all 50 samples with per-sample file links →

Datasets on a similar topic

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