The phosphatase PP1 sustains global transcription by promoting RNA polymerase II pause release [ChIP-seq]

We performed calibrated ChIP-seq, PRO-seq, TT-seq, or RNA-seq to investigate the role of protein phosphotase PNUTS-PP1 in regulating transcription. ChIP-seq, PRO-seq, TT-seq, or RNA-seq were normalizated by MEF as spike-in. The reads passed the quality check aligned to the human hg19 and mouse mm10 genome. In this study, samples for each condition were collected in biological duplicates. Cells were treated with dTAG13 for 3,12h, the corresponding control cells were treated with vehicle only (DMSO) at the same vol/vol dilution.