← BioTransfer GEO Dataset Finder
GEO series

Creb5 controls its own expression and directly induces the joint-interzone regulatory program [RNAseq_Sox9_GFP]

GSE281191 Mus musculus Expression profiling by high throughput sequencing 9 samples Submitted 2025/06/05 Platform GPL24247
Summary
Our prior work indicated that the transcription factor Creb5 plays a crucial role in the genesis of synovial joints. Here, we perform an integrative analysis of the transcriptome, chromatin accessibility, and Creb5-chromatin occupancy in Creb5-expressing synovial joint progenitor cells. This analysis revealed that Creb5 directly binds to both its own two promoters and to the regulatory regions of the early joint markers, Gdf5 and Sfrp2; each of whose expression in the joint-interzone is Creb5-dependent. Functional enhancer analysis indicated that Creb5 binding sites in its own two promoters are necessary for these sequences to drive transgene expression in a pattern that mimics endogenous Creb5 expression. While Creb5 directly regulates both Gdf5 and Sfrp2 expression in the inner joint-interzone by binding to either CREs or TREs in their regulatory elements, Creb5 activates Barx1 expression specifically in the outer joint-interzone; and thus initiates both the formation of the joint-interzone and regionalization of cell fates within this tissue.
Published in
Creb5 controls its own expression and directly induces the joint interzone regulatory program
Zhang CH, Shahini A, Cook LE et al. · Proceedings of the National Academy of Sciences of the United States of America 2025 · PMID 40472036 · doi:10.1073/pnas.2501830122
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE281191_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 9 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1182706 and SRA study SRP543643. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 9 more — browse all 9 samples with per-sample file links →

Similar datasets

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.