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Effects of OGT-ISS deletion on gene expression in NK92 cells

GSE281387 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2026/06/10 Platform GPL24676
Summary
O-GlcNAcylation, a type of post-translational modification, is known to regulate the functions of various immune cells. In this study, we report that increasing O-GlcNAcylation enhances the tumor-killing efficacy of NK cells. By removing the ISS (intronic splicing silencer) of OGT in NK92 cells, we achieved stabilized O-GlcNAcylation even under tumor microenvironment (TME)-mimicking conditions, which led to a heightened killing effect against tumors. To investigate how OGT-ISS deletion impacts gene expression profiles associated with NK cell activity, we performed RNA sequencing (RNA-seq) to compare the global transcriptomes of wild-type (WT) and ΔOGT-ISS_NK92 cells. This comparative transcriptome analysis aims to identify gene expression profiles that influence NK cell cytotoxicity and functional responses in the context of tumor immunity.
Published in
Genetic manipulation of OGT enhances NK cell-mediated cytotoxicity in tumor immunity
Oh SC, Jeon BC, Jang IH et al. · Journal of advanced research 2026 · PMID 40914422 · doi:10.1016/j.jare.2025.08.062
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Also filed as BioProject PRJNA1183781 and SRA study SRP543989. Searching any of these in the dataset finder brings you back here.

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