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LBR and LAP2 mediate heterochromatin tethering to the nuclear periphery to preserve genome homeostasis

GSE281919 Mus musculus (mouse) ChIP-seq / ATAC-seq (genome binding and open chromatin) 111 samples Submitted 2025/10/01 Platform GPL21103Platform GPL24676Platform GPL34328Platform GPL34281Platform GPL20301
At a glance

ChIP-seq / ATAC-seq (genome binding and open chromatin) · mouse · 111 samples

What it is
ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
Organism
Mus musculus (mouse)
Samples
111 samples: 29 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
LBR and LAP2 mediate heterochromatin tethering to the nuclear periphery to preserve genome homeostasisNature cell biology · 2026
Summary (written by the submitters)
In most eukaryotic cells, euchromatin is localized in the nuclear interior, whereas heterochromatin is enriched at the nuclear envelope (NE). This conventional chromatin organization is established by heterochromatin tethering to the NE, however its importance for cellular homeostasis is largely unexplored. Peripheral heterochromatin localization relies on redundant NE-tethering systems.
Read the full summary ↓
One tether is constituted by the lamin B receptor (LBR) in mammals, but the enigmatic nature of the other tethers has hampered functional analyses. Here we demonstrate that the downregulation of abundant, ubiquitous NE proteins can induce the global detachment of heterochromatin from the NE. Among these factors, we identify LBR and LAP2 as major players in bulk heterochromatin attachment to the NE in pluripotent and differentiated mammalian cells. Their loss leads to repositioning of heterochromatin to the nuclear interior, changes in chromatin accessibility, deregulation of gene expression including activation of antiviral innate immunity, and defects in cell fate determination.
Published in
LBR and LAP2 mediate heterochromatin tethering to the nuclear periphery to preserve genome homeostasis
Lewis R, Sinigiani V, Maziak N et al. · Nature cell biology 2026 · PMID 41735607 · doi:10.1038/s41556-025-01822-7
This dataset
Open GSE281919 in finder → Find more RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE281919_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.

Also filed as BioProject PRJNA1186175 and SRA study SRP545285. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41735607) ↗
Study design
29 conditions, mostly in triplicate
mESC_WT ×4 hct116_dko ×4 hct116_wt ×4 HCT116_ctrl ×3 HCT116_si11 ×3 HCT116_LBR_LMNA_KO_HLLad7 ×3 HCT116_LBR_TMPO_KO_HLTd3 ×3 HCT116_LBR_TMPO_LMNA_KO_HLTLad5 ×3 +45 more

Supports a between-group comparison across 87 samples.

How we read this: 29 replicated groups read from 111 sample titles; they account for 87 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 71 more — browse all 111 samples with per-sample file links →

Datasets on a similar topic

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