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SASP Suppression by hTSCs Secretome

GSE282054 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2026/01/21 Platform GPL34284
Summary
Cellular senescence, a state of irreversible cell cycle arrest, contributes to aging and age-related diseases through the accumulation of senescent cells and their secretion of a pro-inflammatory senescence-associated secretory phenotype (SASP).Recent studies indicated that extracellular vesicles (EVs) as key SASP components mediating intercellular communication. Here, we investigated the effects of human trophoblast stem cell secretome and EVs (hTSC-S, hTSC-EVs) on cellular senescence. Our results show that hTSC-S and hTSC-EVs reduce SASP-associated mRNAs and cytokines, including CXCL1 and GDF15, and suppress NF-κB activation in senescent WI-38 cells. Proteomic analysis revealed an enrichment of ECM-remodeling proteins in hTSC-EVs, suggesting restorative properties. In vivo, hTSC-S decreased GDF15, CXCL1, and markers of DNA damage in aged mice, underscoring its therapeutic potential to modulate SASP and promote healthier aging.
Published in
The Secretome of Human Trophoblast Stem Cells Attenuates Senescence-Associated Traits
Abdelmohsen K, Martindale JL, Rossi M et al. · Aging cell 2026 · PMID 41521531 · doi:10.1111/acel.70368
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Also filed as BioProject PRJNA1186722 and SRA study SRP545513. Searching any of these in the dataset finder brings you back here.

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