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X-chromosome upregulation operates on a gene-by-gene basis at RNA and protein levels [ChIP-seq]

GSE282785 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 4 samples Submitted 2025/06/19 Platform GPL30172
Summary
Gene dosage compensation mechanisms are crucial for mammalian development. In mice, recent findings show that cells can sense the number of X chromosomes. Loss or inactivation of one of the two X chromosomes is compensated by upregulating the remaining active X chromosome, a process termed X-chromosome upregulation (XCU). However, how cells sense X-chromosome dosage and induce XCU remains unclear. Here, we show that heterozygous X chromosome fragment deletions in mouse pluripotent stem cells induces XCU in trans, and that compensation takes place at the mRNA and protein level. Furthermore, we found that inducing gene silencing in cis on autosomes induces gene dosage compensation in trans. This work provides significant insights into the molecular foundations of mammalian gene dosage compensation.
Published in
X-chromosome upregulation operates on a gene-by-gene basis at RNA and protein levels
Allsop RN, Boeren J, Tan BF et al. · Nature communications 2025 · PMID 41027898 · doi:10.1038/s41467-025-64195-3
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Also filed as BioProject PRJNA1190422 and SRA study SRP547545. Searching any of these in the dataset finder brings you back here.

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