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Synaptotagmin-7 deficit causes insulin hypoactivity and contributes to behavioral alterations in mice

GSE282863 Mus musculus (mouse) RNA-seq (gene expression) 13 samples Submitted 2025/05/14 Platform GPL23479
At a glance

RNA-seq (gene expression) · mouse · 13 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
13 samples: 3 × Hippocampus, Light phase, WT, vehicle, 30min, vs 3 × Hippocampus, Light phase, WT, BMS, 30min, vs 3 × Hippocampus, Dark phase, WT, vehicle, 30min, vs 2 × Hippocampus, Light phase, Syt7 KO, vehicle, 30min,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Synaptotagmin-7 deficit causes insulin hypoactivity and contributes to behavioral alterations in miceiScience · 2025
Summary (written by the submitters)
Synaptotagmin-7 (Syt7) KO mice show diurnal fluctuations of mania- and depression-like behavioral abnormalities. Although GluN2B-NMDAR hypoactivity has been shown to be involved in the induction of mania-like behaviors of the Syt7 KO mice in the dark phase, the reasons for the depression-like behaviors in the light phase and behavioral fluctuation remain unknown.
Read the full summary ↓
Here, we show that bipolar I disorder (BDI)-patient-induced pluripotent stem cell (iPSC)-derived islet-like organoids exhibited Syt7-dependent insulin secretion defects; moreover, Syt7-deficiency-induced insulin hyposecretion generated depression-like behaviors in Syt7 KO mice in the light phase. Furthermore, pancreatic insulin secretion and neuronal activity showed opposite diurnal patterns, in which the Syt7-deficiency-induced disequilibrium induced periodic antagonistic shifts in the mania- and depression-like behaviors. Finally, using RNA sequencing (RNA-seq) analysis, we explored downstream pathways that might underlie the diurnal fluctuation of behaviors. Therefore, Syt7-deficiency-induced insulin hypoactivity contributed to light-phase depression-like behaviors and diurnal behavioral fluctuations in the mice.
Published in
Synaptotagmin-7 deficit causes insulin hypoactivity and contributes to behavioral alterations in mice
Liu YN, Wang QW, Lu SY et al. · iScience 2025 · PMID 40330888 · doi:10.1016/j.isci.2025.112354
This dataset
Open GSE282863 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE282863_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1190907 and SRA study SRP547761. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40330888) ↗
Study design
3 × Hippocampus, Light phase, WT, vehicle, 30min, vs 3 × Hippocampus, Light phase, WT, BMS, 30min, vs 3 × Hippocampus, Dark phase, WT, vehicle, 30min, vs 2 × Hippocampus, Light phase, Syt7 KO, vehicle, 30min,

Supports a between-group comparison across 11 samples.

How we read this: 4 replicated groups read from 13 sample titles; they account for 11 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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