GEO series

Gpr37 contributes to inflammation-induced GI dysmotility by regulating enteric reactive gliosis.

GSE262418 Mus musculus (mouse) RNA-seq (gene expression) 75 samples Submitted 2025/06/24 Platform GPL21626

At a glance

RNA-seq (gene expression) · mouse · 75 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 75 samples: 16 conditions, mostly with about 4 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Gpr37 modulates the severity of inflammation-induced GI dysmotility by regulating enteric reactive gliosisiScience · 2025

Summary (written by the submitters)

The enteric nervous system (ENS) is contained within two layers of the gut wall and is made up of neurons, immune cells, and enteric glia cells (EGCs) that regulate gastrointestinal (GI) function. EGCs in both inflammatory bowel disease (IBD) and irritable bowel syndrome (IBS) change in response to inflammation, referred to as reactive gliosis.

Read the full summary ↓

Whether EGCs restricted to a specific layer or region within the GI tract alone can influence intestinal immune response is unknown. Using bulk RNA-sequencing and in situ hybridization, we identify G-protein coupled receptor, Gpr37, as a gene expressed only in EGCs of the myenteric plexus, one of the two layers of the ENS. We show that Gpr37 contributes to key components of LPS-induced reactive gliosis including activation of NF-kB and IFN-y signaling and response genes, lymphocyte recruitment, and inflammation-induced GI dysmotility. Targeting Gpr37 in EGCs presents a potential avenue for modifying inflammatory processes in the ENS.

Published in

Gpr37 modulates the severity of inflammation-induced GI dysmotility by regulating enteric reactive gliosis

Robertson K, Hahn O, Tantry A et al. · iScience 2025 · PMID 40678517 · doi:10.1016/j.isci.2025.112885

This dataset

Open GSE262418 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE262418_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1091683 and SRA study SRP497744. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40678517) ↗

Study design

16 conditions, mostly with about 4 replicates each

EGC, None, SI_MP, ×6 EGC, None, SI_SMP, ×6 EGC, PBS, WT, SI, 24hr, ×6 EGC, None, Colon_MP, ×5 EGC, None, Colon_SMP, ×5 EGC, LPS, KO, SI, 24hr, ×5 EGC, PBS, KO, Colon, 24hr, ×5 EGC, PBS, Colon_MP, 2hr, ×4 +9 more

Supports a between-group comparison across 74 samples.

How we read this: 16 replicated groups read from 75 sample titles; they account for 74 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM8168439 EGC, None, Colon_MP, rep1 EGC, None, Colon_MP,
GSM8168441 EGC, None, Colon_MP, rep2 EGC, None, Colon_MP,
GSM8168442 EGC, None, Colon_MP, rep3 EGC, None, Colon_MP,
GSM8168443 EGC, None, SI_MP, rep1 EGC, None, SI_MP,
GSM8168444 EGC, None, SI_MP, rep2 EGC, None, SI_MP,
GSM8168446 EGC, None, SI_MP, rep3 EGC, None, SI_MP,
GSM8168447 EGC, None, Colon_SMP, rep1 EGC, None, Colon_SMP,
GSM8168448 EGC, None, Colon_SMP, rep2 EGC, None, Colon_SMP,
GSM8168449 EGC, None, SI_SMP, rep1 EGC, None, SI_SMP,
GSM8168451 EGC, None, SI_SMP, rep2 EGC, None, SI_SMP,
GSM8168452 EGC, None, SI_SMP, rep3 EGC, None, SI_SMP,
GSM8168453 EGC, None, Colon_MP, rep4 EGC, None, Colon_MP,
GSM8168455 EGC, None, Colon_MP, rep5 EGC, None, Colon_MP,
GSM8168456 EGC, None, SI_MP, rep4 EGC, None, SI_MP,
GSM8168457 EGC, None, SI_MP, rep5 EGC, None, SI_MP,
GSM8168458 EGC, None, SI_MP, rep6 EGC, None, SI_MP,
GSM8168460 EGC, None, Colon_SMP, rep4 EGC, None, Colon_SMP,
GSM8168461 EGC, None, Colon_SMP, rep5 EGC, None, Colon_SMP,
GSM8168462 EGC, None, Colon_SMP, rep6 EGC, None, Colon_SMP,
GSM8168463 EGC, None, SI_SMP, rep4 EGC, None, SI_SMP,
GSM8168465 EGC, None, SI_SMP, rep5 EGC, None, SI_SMP,
GSM8168466 EGC, None, SI_SMP, rep6 EGC, None, SI_SMP,
GSM8168467 EGC, PBS, Colon_MP, 2hr, rep1 EGC, PBS, Colon_MP, 2hr,
GSM8168468 EGC, PBS, Colon_MP, 2hr, rep2 EGC, PBS, Colon_MP, 2hr,
GSM8168470 EGC, PBS, Colon_MP, 2hr, rep3 EGC, PBS, Colon_MP, 2hr,
GSM8168471 EGC, PBS, Colon_MP, 2hr, rep4 EGC, PBS, Colon_MP, 2hr,
GSM8168472 EGC, PBS, SI_MP, 2hr, rep1 EGC, PBS, SI_MP, 2hr,
GSM8168474 EGC, PBS, SI_MP, 2hr, rep2 EGC, PBS, SI_MP, 2hr,
GSM8168475 EGC, PBS, SI_MP, 2hr, rep3 EGC, PBS, SI_MP, 2hr,
GSM8168476 EGC, PBS, SI_MP, 2hr, rep4 EGC, PBS, SI_MP, 2hr,
GSM8168477 EGC, LPS, Colon_MP, 2hr, rep1 EGC, LPS, Colon_MP, 2h…
GSM8168479 EGC, LPS, Colon_MP, 2hr, rep2 EGC, LPS, Colon_MP, 2h…
GSM8168480 EGC, LPS, Colon_MP, 2hr, rep3 EGC, LPS, Colon_MP, 2h…
GSM8168481 EGC, LPS, Colon_MP, 2hr, rep4 EGC, LPS, Colon_MP, 2h…
GSM8168482 EGC, LPS, SI_MP, 2hr, rep1 EGC, LPS, SI_MP, 2hr,
GSM8168484 EGC, LPS, SI_MP, 2hr, rep2 EGC, LPS, SI_MP, 2hr,
GSM8168485 EGC, LPS, SI_MP, 2hr, rep3 EGC, LPS, SI_MP, 2hr,
GSM8168486 EGC, LPS, SI_MP, 2hr, rep4 EGC, LPS, SI_MP, 2hr,
GSM8168487 EGC, LPS, WT, Colon, 24hr, rep1 EGC, LPS, WT, Colon, 24…
GSM8168489 EGC, LPS, WT, Colon, 24hr, rep2 EGC, LPS, WT, Colon, 24…

+ 35 more — browse all 75 samples with per-sample file links →

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