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Ultra high-throughput single-cell transcriptome and epitope profiling of healthy human PBMCs using SCITO-seq2

GSE282881 Homo sapiens Expression profiling by high throughput sequencing; Other 4 samples Submitted 2026/01/04 Platform GPL24676
Summary
This study presents comprehensive single-cell transcriptome and surface protein profiling using SCITO-seq2, an enhanced platform that improves the recovery of unique transcripts while enabling simultaneous detection of surface proteins. The analysis was conducted in two phases: first, using healthy donor PBMCs to validate technical performance, and second, by comparing samples from healthy controls, patients with childhood systemic lupus erythematosus (cSLE), and patients with CTLA4 haploinsufficiency and autoimmune infiltration (CHAI). These results demonstrate the platform’s applicability to both normal and disease states.
Published in
SCITO-seq2: ultra-high-throughput single-cell transcriptome and epitope sequencing
Lee SH, Jin BY, Lee CR et al. · Genome biology 2026 · PMID 41593779 · doi:10.1186/s13059-026-03954-x
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Also filed as BioProject PRJNA1190934 and SRA study SRP547723. Searching any of these in the dataset finder brings you back here.

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