← BioTransfer GEO Dataset Finder
GEO series

Single cell landscape of MBL/CLL transition [MBL_CLL_GEO_DataSet3]

GSE295491 Homo sapiens (human) Single-cell RNA-seq 62 samples Submitted 2026/03/31 Platform GPL24676
At a glance

Single-cell RNA-seq · human · 62 samples

What it is
Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart.
Organism
Homo sapiens (human)
Topic
Chronic lymphocytic leukemiaThis series is part of our curated briefing on it.
Samples
62 samples: 62 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Single-cell epigenetic and transcriptomic states across the continuum of monoclonal B cell lymphocytosis to chronic lymphocytic leukemiaGenome biology · 2026
Summary (written by the submitters)
The transition of monoclonal B cell lymphocytosis (MBL) to chronic lymphocytic leukemia (CLL) remains ill-defined at the epigenetic and transcriptional level. Here, we performed single cell RNA and ATAC sequencing of low-count (LC) and high-count (HC) MBL and CLL blood samples to track the continuum from physiologic B cells to CLL.
Published in
Single-cell epigenetic and transcriptomic states across the continuum of monoclonal B cell lymphocytosis to chronic lymphocytic leukemia
Rathgeber AC, Fernandes SM, Nagler A et al. · Genome biology 2026 · PMID 41987205 · doi:10.1186/s13059-026-04072-4
This dataset
Open GSE295491 in finder → Find more human RNA-seq datasets → Chronic lymphocytic leukemia landscape →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE295491_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA1254962 and SRA study SRP581191. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41987205) ↗
Study design
62 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 62 sample titles: 62 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 22 more — browse all 62 samples with per-sample file links →

More Chronic lymphocytic leukemia datasets

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.