GEO series

Differential behavior responses and genetic alteration underpinning exercise effectiveness in stress-susceptible mice

GSE283250 Mus musculus (mouse) RNA-seq (gene expression) 12 samples Submitted 2024/12/04 Platform GPL24247

At a glance

RNA-seq (gene expression) · mouse · 12 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 12 samples: 12 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Differential behavior responses and genetic alteration underpinning exercise effectiveness in stress-susceptible miceScientific reports · 2025

Summary (written by the submitters)

Stress susceptibility varies across individuals, influenced by genetic, molecular, and environmental factors. Considering the individual differences in response to stress, however, approaches for exercise treatment as an antidepressant and anxiolytic intervention are also not exempt from variability in effectiveness among individuals, and insight into the biology of those variations can be gained by examining the individual adaptation to exercise.

Read the full summary ↓

Here, we investigated the efficacy of voluntary wheel running (VWR) exercise as a disease-modifying intervention for stress-susceptible (SS) mice subjected to chronic restraint stress (CRST). Multidimensional behavior analysis revealed significant variability in the efficacy of VWR among individuals, with some mice showing substantial behavior phenotypic improvements (e.g., behavior despair, anhedonia, aversion to open space, and compulsive behavior) (SES) while others displayed limited or no benefits (SER). Furthermore, transcriptomic analysis of the ventral hippocampus (vHPC), a critical brain region involved in emotional regulation and processing, demonstrated that SES mice exhibited molecular signatures promoting adaptive changes by restoring cellular repair, energy availability, and synaptic plasticity observed in SS mice, whereas SER mice exhibited limited behavior resilience and distinct transcriptomic profiles enriched in structural adaptation without functional resilience and glia cell differentiation, particularly marked by astrocyte activation or differentiation. These findings suggest that while VWR can alleviate multiple concurrent behavior symptoms in stress-susceptible mice; however, its efficacy is influenced by distinct biological processes. Collectively, our work highlights the importance of a multivariate framework for behavior assessment and genetic underpinnings to better understand the often-overlooked variability in response to stress and the therapeutic efficacy of exercise in stress-related disorders.

Published in

Differential behavior responses and genetic alteration underpinning exercise effectiveness in stress-susceptible mice

Hwang DJ, Kim JE, Cho JY et al. · Scientific reports 2025 · PMID 40295575 · doi:10.1038/s41598-025-98630-8

This dataset

Open GSE283250 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE283250_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1192959 and SRA study SRP548627. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40295575) ↗

Study design

12 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 12 sample titles: 12 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM8658642 C57BL/6J mice (no. 2)
GSM8658643 C57BL/6J mice (no. 8)
GSM8658644 C57BL/6J mice (no. 12)
GSM8658645 C57BL/6J mice (no. 6), Stress-susceptible mice, Voluntary wheel running 14d(Ineffective response to VWR)
GSM8658646 C57BL/6J mice (no. 19), Stress-susceptible mice, Voluntary wheel running 14d(Ineffective response to VWR)
GSM8658647 C57BL/6J mice (no. 20), Stress-susceptible mice, Voluntary wheel running 14d(Ineffective response to VWR)
GSM8658648 C57BL/6J mice (no. 9), Stress-susceptible mice, Voluntary wheel running 14d(Effective response to VWR)
GSM8658649 C57BL/6J mice (no. 10), Stress-susceptible mice, Voluntary wheel running 14d(Effective response to VWR)
GSM8658650 C57BL/6J mice (no. 12), Stress-susceptible mice, Voluntary wheel running 14d(Effective response to VWR)
GSM8658651 C57BL/6J mice (no. 18), Restraint stress 14d(Stress-susceptible mice)
GSM8658652 C57BL/6J mice (no. 19), Restraint stress 14d(Stress-susceptible mice)
GSM8658653 C57BL/6J mice (no. 21), Restraint stress 14d(Stress-susceptible mice)

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.