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THAP1 is a maternal effect factor required for the first cell cycle via Rrm1 in early mouse embryos

GSE283275 Mus musculus (mouse) RNA-seq (gene expression) 44 samples Submitted 2025/12/19 Platform GPL28330
At a glance

RNA-seq (gene expression) · mouse · 44 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
44 samples: 18 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
THAP1 is a maternal effect factor required for the first cell cycle via Rrm1 in early mouse embryosEMBO reports · 2026
Summary (written by the submitters)
Maternal effect genes (MEGs) produce factors that accumulate in oocytes and play critical roles in embryo development. Mutations of MEGs are frequently linked to reproductive and congenital disorders. The majority of identified mammalian MEGs encode epigenetic factors and RNA regulators. Here, we identify a MEG encoding the transcription factor Thanatos-associated protein 1 (Thap1).
Read the full summary ↓
Thap1 is highly expressed in mouse oocytes and early embryos. Oocyte-specific deletion of Thap1 results in delayed progression of mouse embryos from the 1-cell to the 2-cell stage and 2-cell arrest, accompanied by defective zygotic genome activation (ZGA) and strongly impaired female fertility. Mechanistically, THAP1 activates a critical subset of genes in oocytes, including Rrm1, which produces ribonucleotide reductase required for generating deoxynucleotide triphosphates (dNTPs). Low-input metabolome profiling across 7 stages during the oocyte-to-embryo transition shows gradual, THAP1-dependent dNTP accumulation that peaks in MII oocytes. Overexpression of Rrm1 in zygotes almost fully restores the 2-cell progression and ZGA in Thap1 maternal-knockout embryos. Our findings identify THAP1 as a key maternal effector critical for the earliest stage of mammalian development.
Published in
THAP1 is a maternal effect factor required for the first cell cycle via Rrm1 in early mouse embryos
Fan Q, Wu X, Dang Y et al. · EMBO reports 2026 · PMID 41731150 · doi:10.1038/s44319-026-00712-9
This dataset
Open GSE283275 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE283275_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1193005 and SRA study SRP548732. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41731150) ↗
Study design
18 conditions, mostly in duplicate
Ctrl_PN5_1C_embryo_RNA ×4 Thap1_mKO_PN5_1C_embryo_RNA ×4 Ctrl_D14_oocyte_RNA ×2 Thap1_mKO_D14_oocyte_RNA ×2 Ctrl_D60_FGO_RNA ×2 Thap1_mKO_D60_FGO_RNA ×2 Ctrl_MII_oocyte_RNA ×2 Thap1_mKO_MII_oocyte_RNA ×2 +14 more

Supports a between-group comparison across 40 samples.

How we read this: 18 replicated groups read from 44 sample titles; they account for 40 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 4 more — browse all 44 samples with per-sample file links →

Datasets on a similar topic

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