← BioTransfer GEO Dataset Finder
GEO series

HUMAN ALVEOLAR MACROPHAGE FUNCTION IS IMPAIRED IN TUBERCULOSIS CONTACTS WITH DIABETES

GSE283452 Homo sapiens (human) RNA-seq (gene expression) 89 samples Submitted 2025/12/31 Platform GPL21290
At a glance

RNA-seq (gene expression) · human · 89 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
89 samples: 18 conditions, mostly with about 5 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Human alveolar macrophage function is impaired in tuberculosis contacts with diabetesEBioMedicine · 2025
Summary (written by the submitters)
Patients with type 2 diabetes (T2D) are more susceptible to Mycobacterium tuberculosis (M.tb) infection and severe tuberculosis (TB). The underlying mechanisms contributing to this remain largely unknown. To fill this critical knowledge gap, we obtained human alveolar macrophages (HAMs) and monocyte-derived macrophages (MDMs) from TB-exposed individuals with and without T2D in South Africa. We infected HAMs and MDMs ex vivo with live M.tb then collected RNA for RNAseq after 2, 24, 72h.
Published in
Human alveolar macrophage function is impaired in tuberculosis contacts with diabetes
Kleynhans L, Kunsevi-Kilola C, Tshivhula H et al. · EBioMedicine 2025 · PMID 41330113 · doi:10.1016/j.ebiom.2025.106050
This dataset
Open GSE283452 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE283452_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1193683 and SRA study SRP549153. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41330113) ↗
Study design
18 conditions, mostly with about 5 replicates each
T2D_HAM_2h -Mtb biol ×8 noT2D_HAM_2h +Mtb biol ×8 T2D_HAM_2h +Mtb biol ×7 T2D_HAM_24h -Mtb biol ×6 T2D_HAM_24h +Mtb biol ×6 T2D_MDM_2h +Mtb biol ×6 T2D_HAM_72h +Mtb biol ×5 T2D_MDM_2h -Mtb biol ×5 +10 more

Supports a between-group comparison across 89 samples.

How we read this: 18 replicated groups read from 89 sample titles; they account for 89 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 49 more — browse all 89 samples with per-sample file links →

Datasets on a similar topic

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.