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TSLP acts on regulatory T cells to maintain their identity and limit allergic inflammation

GSE283565 Mus musculus (mouse) RNA-seq (gene expression) 10 samples Submitted 2024/12/25 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 10 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
10 samples: 3 × Treg from Foxp3YFP-Cre vs 3 × Treg from Crlf2fl/fl Foxp3YFP-Cre vs 2 × nTregs cultured for 24h with TCR vs 2 × nTregs cultured for 24h with TCR+TSLPGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
TSLP acts on regulatory T cells to maintain their identity and limit allergic inflammationScience immunology · 2025
Summary (written by the submitters)
Thymic stromal lymphopoietin (TSLP) is a type I cytokine that promotes allergic responses and mediates type 2 immunity. A balance between effector T cells (Teff), which drive the immune response, and regulatory T cells (Tregs), which suppress the response, is required for proper immune homeostasis. Here, we report that TSLP differentially acts on Teff versus Tregs to balance type 2 immunity.
Read the full summary ↓
As expected, deletion of TSLPR on all T cells (Cd4CreCrlf2fl/fl mice) resulted in lower numbers of Th2 cells and diminished ovalbumin-induced airway inflammation, but selective deletion of TSLPR on Tregs (Foxp3YFP-Cre/YCrlf2fl/fl mice) resulted in increased IL-5- and IL-13-secreting Th2 cells and lung eosinophilia. Moreover, TSLP augmented the expression of factors that stabilize Tregs. During type 2 immune responses, TSLPR-deficient Tregs acquired Th2-like properties, with augmented GATA3 expression and secretion of IL-13. TSLP is not only a driver of Th2 effector cells but also acts in a negative feedback loop, thus promoting the ability of Tregs to limit allergic inflammation.
Published in
TSLP acts on regulatory T cells to maintain their identity and limit allergic inflammation
Gurram RK, Li P, Oh J et al. · Science immunology 2025 · PMID 39792638 · doi:10.1126/sciimmunol.adk0073
This dataset
Open GSE283565 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE283565_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1194534 and SRA study SRP549581. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 39792638) ↗
Study design
3 × Treg from Foxp3YFP-Cre vs 3 × Treg from Crlf2fl/fl Foxp3YFP-Cre vs 2 × nTregs cultured for 24h with TCR vs 2 × nTregs cultured for 24h with TCR+TSLP

Supports a between-group comparison across 10 samples.

How we read this: 4 replicated groups read from 10 sample titles; they account for 10 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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