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Tcf1 and Lef1 transcription factors underpin the inception of T cell fate

GSE283601 Mus musculus Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing 8 samples Submitted 2025/09/17 Platform GPL24247
Summary
The goal of this study is to resolve the heterogeneity of transcriptomic and chromatin accessibility (ChrAcc) landscape in DN1 thymocytes harboring ETPs. Comparison of molecular features between ETPs with non-ETP DN1 cells predicted contribution by Tcf/Lef family transcription factors, and pre-thymic ablation of Tcf1 and Lef1 impaired ETP formation. Single cell analysis of the factor-targeted ETPs revealed that they converged on Notch1 or Notch pathway effector molecules including Hes1 and Hhex, to support their transcriptional activation.
Published in
Single-cell multiomics identifies Tcf1 and Lef1 as key initiators of early thymic progenitor fate
Zhao X, Hu SS, Lee WH et al. · Science immunology 2025 · PMID 40938954 · doi:10.1126/sciimmunol.adq8970
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Also filed as BioProject PRJNA1194599 and SRA study SRP549636. Searching any of these in the dataset finder brings you back here.

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