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Targeted Apoptosis of the Angiotensin AT1R (Agtr1a)-Expressing Subtype of Agouti-Related Peptide Neurons in Adult Mice Increases Food Intake and Resting Metabolism, and Alters Blood Pressure Control

GSE284096 Mus musculus (mouse) RNA-seq (gene expression) 24 samples Submitted 2025/06/01 Platform GPL34290
At a glance

RNA-seq (gene expression) · mouse · 24 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
24 samples: 6 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Angiotensin AT(1)R expressing cells within the ventral hypothalamus modulate integrative control of cardiometabolic functionsiScience · 2025
Summary (written by the submitters)
Background: Neurons of the hypothalamic arcuate nucleus that express Agouti-related peptide (AgRP) have been implicated in control of energy intake and expenditure. Recently we discovered that subtypes of AgRP neurons can be distinguished based upon expression of angiotensin II receptors. The Type 1 subtype is inhibited via the angiotensin type 1 receptor (AT1R / Agtr1a).
Read the full summary ↓
We hypothesized that ablation of Type 1 AgRP neurons would have long-term consequences on energy balance. Methods: Young adult mice expressing Cre-recombinase via the Agtr1a locus received bilateral microinjection of an AAV vector encoding Cre-dependent expression of caspase-3 into the arcuate nucleus. An array of cardiometabolic phenotypes were then serially evaluated in mice housed at 22°C or 30°C. Results: Ablation of Type 1 AgRP neurons caused increased food intake, adiposity, resting metabolic rate via a mechanism involving hepatic function and nutrient partitioning, increased preferred ambient temperature, progressive loss of core temperature defense, and changes in the relationship between blood pressure and heart rate. Many phenotypes were ameliorated by housing animals at thermoneutrality. Conclusions: The Type 1 AgRP neuron influences cardiometabolic control through modulation of thermoregulatory responses to cold. Some functions of Type 1 AgRP neurons appear to oppose canonical roles of other AgRP neuron subtypes.
Published in
Angiotensin AT(1)R expressing cells within the ventral hypothalamus modulate integrative control of cardiometabolic functions
Pasos J, Wagner VA, Lawton SBR et al. · iScience 2025 · PMID 40585361 · doi:10.1016/j.isci.2025.112797
This dataset
Open GSE284096 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE284096_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1197013 and SRA study SRP550957. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40585361) ↗
Study design
6 conditions, mostly in triplicate
Ai39 x BAC-AT1A-Cre, Male, ablated treatment,… ×3 Ai39 x BAC-AT1A-Cre, Male, control treatment,… ×3 Ai39 x BAC-AT1A-Cre, Female, ablated treatmen… ×3 Ai39 x BAC-AT1A-Cre, Female, control treatmen… ×3 Ai39 x BAC-AT1A-Cre, Female, control treatmen… ×3 Ai39 x BAC-AT1A-Cre, Female, ablated treatmen… ×2 Ai39 x BAC-AT1A-Cre, Male, ablated treatment,… ×1 Ai9 x BAC-AT1A-Cre, Male, ablated treatment, … ×1 +5 more

Supports a between-group comparison across 17 samples.

How we read this: 6 replicated groups read from 24 sample titles; they account for 17 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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